mouse monoclonal antibodies against human cd26  (Thermo Fisher)

Journal:

Article Title: Co-Purification of Mac-2 Binding Protein with Galectin-3 and Association with Prostasomes in Human Semen

doi: 10.1002/pros.21287

Figure Lengend Snippet: Peptide Matches from Tandem Mass Spectrometry of Proteins Purified by Lactose Affinity Chromatography of Human Prostasomes

Article Snippet: Mouse monoclonal antibodies against human CD26 were from Lab Vision (Fremont, CA).

Techniques: Mass Spectrometry, Purification, Affinity Chromatography, Sequencing, Binding Assay

Journal:

Article Title: Co-Purification of Mac-2 Binding Protein with Galectin-3 and Association with Prostasomes in Human Semen

doi: 10.1002/pros.21287

Figure Lengend Snippet: Protein-protein interaction network of the identified proteins. Cytoscape was used to display the protein-protein interaction map generated from interaction database data pulled down from the HPRD, BioGRID, IntAct, and NCI/Nature Pathway Interaction databases. White nodes indicate the proteins identified in this study following β-galactoside affinity purification. Gray nodes indicate interacting proteins retrieved from the databases. Connecting lines indicate identified protein interactions. Each protein is shown by its gene symbol or entrez identification number. Galectin-3 (LGALS3), M2BP (LGALS3BP), lactoferrin (LTF), PIP (PIP), CD26 (DPP4), seminogelin I (SEMG1), seminogelin 2 (SEMG2), olfactomedin 4 (OLFM4).

Article Snippet: Mouse monoclonal antibodies against human CD26 were from Lab Vision (Fremont, CA).

Techniques: Generated, Affinity Purification

Journal:

Article Title: Co-Purification of Mac-2 Binding Protein with Galectin-3 and Association with Prostasomes in Human Semen

doi: 10.1002/pros.21287

Figure Lengend Snippet: Prostasome purification by size exclusion chromatography and M2BP immunoblot analysis. The membrane fraction from seminal plasma was subjected to size exclusion column chromatography on Sephacryl S300 and prostasomes were collected in the void volume (fractions 14-19). Electroblots of collected fractions were stained for total protein with Ponceau S and for M2BP (R&D polyclonal antibodies) and CD26 immunoreactivity. Molecular weight markers are indicated in kDa.

Article Snippet: Mouse monoclonal antibodies against human CD26 were from Lab Vision (Fremont, CA).

Techniques: Purification, Size-exclusion Chromatography, Western Blot, Column Chromatography, Staining, Molecular Weight